89k - An Overview

89k - An Overview

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shuttle plasmid (pSET1 or pVA838) build that contains the purposeful copy on the mutated gene fused in-frame into a cloned S. suis

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2 that doesn't In a natural way consist of 89K, was engineered for being chloramphenicol-resistant by changing the recA

Affirmation of this hypothesis was obtained via the observation that transfer of the SpcR-marked 89K happened at a frequency of around ten−six for every donor, just like that in the Pseudomonas clc

The amplification specificity of each PCR was verified by pinpointing melting curves and by electrophoresis of the ultimate PCR items, and effectiveness was resolute making use of linearized pMDph plasmid DNA, which contains an individual duplicate of attP

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These observations recommend that the 89K-encoded integrase and excisionase likely cooperatively create the extrachromosomal circular sort of 89K, which signifies an intermediate needed for your transfer method itself.

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MobA89K and its N-terminal relaxase domain Screen unique nicking activity during the presence with the MobC89K accent protein in vitro

is entirely or mainly determined inside the GIs of the above-stated Gram-destructive pathogens, suggesting that GI-variety T4SSs are very conserved among these species (Juhas et al

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did not absolutely abolish the conjugal transfer potential of 89K. VirB6-89K is really a highly hydrophobic protein containing 6 predicted transmembrane segments and is the best prospect for just a channel-forming protein in A. tumefaciens

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